"Activography": a novel, versatile and easily adaptable method for monitoring enzymatic activities in situ

We developed activography to map enzymatic activities on tissue sections using activity-based probes. The assay was validated using a new protease-activity probe on skin biopsies to provide proof-of-concept. Activography is more selective and technically easier than the established in situ zymograph...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2017-03, Vol.53 (22), p.3246-3248
Hauptverfasser:	Pampalakis, G, Zingkou, E, Vekrellis, K, Sotiropoulou, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Assaying Biotin - chemistry Biotin - metabolism Kallikreins - deficiency Kallikreins - metabolism Mice Mice, Knockout Molecular Probes - chemical synthesis Molecular Probes - chemistry Molecular Probes - metabolism Molecular Structure Monitoring Organophosphonates - chemistry Organophosphonates - metabolism Routines Running Serine Peptidase Inhibitor Kazal-Type 5 - deficiency Serine Peptidase Inhibitor Kazal-Type 5 - metabolism Skin (anatomy) Skin - metabolism
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Beschreibung
Zusammenfassung:	We developed activography to map enzymatic activities on tissue sections using activity-based probes. The assay was validated using a new protease-activity probe on skin biopsies to provide proof-of-concept. Activography is more selective and technically easier than the established in situ zymography, thus, adaptable in routine running clinico-chemical laboratories.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c7cc01081h