Selection of aptamers targeting the sialic acid receptor of hemagglutinin by epitope-specific SELEX

A new SELEX scheme is proposed for the selection of aptamers targeting a specific epitope of a native protein. Anti-sialic acid receptor (SAR) aptamers that inhibit H1 hemagglutination at a low picomole dose are selected accordingly.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2014-08, Vol.50 (63), p.8719-8722
Hauptverfasser:	Lao, Yeh-Hsing, Chiang, Hui-Yu, Yang, Deng-Kai, Peck, Konan, Chen, Lin-Chi
Format:	Artikel
Sprache:	eng
Schlagworte:	Antiviral Agents - chemical synthesis Antiviral Agents - pharmacology Aptamers, Nucleotide - genetics DNA, Single-Stranded - chemistry Epitopes - drug effects Hemagglutination - drug effects Hemagglutinins - drug effects Humans Influenza A Virus, H1N1 Subtype - drug effects Proteins Receptors Receptors, Cell Surface - drug effects SELEX Aptamer Technique Structure-Activity Relationship
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Beschreibung
Zusammenfassung:	A new SELEX scheme is proposed for the selection of aptamers targeting a specific epitope of a native protein. Anti-sialic acid receptor (SAR) aptamers that inhibit H1 hemagglutination at a low picomole dose are selected accordingly.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c4cc03116d