Membrane analysis with amphiphilic carbon dots

Membrane analysis with amphiphilic carbon dots

Newly-synthesized amphiphilic carbon dots were used for spectroscopic analysis and multicolour microscopic imaging of membranes and live cells. We show that Förster resonance energy transfer (FRET) occurred from the amphiphilic carbon dots to different membrane-associated fluorescence acceptors. The...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2014-09, Vol.50 (71), p.10299-10302
Hauptverfasser: Nandi, Sukhendu, Malishev, Ravit, Parambath Kootery, Kaviya, Mirsky, Yelena, Kolusheva, Sofiya, Jelinek, Raz
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Carbon
Carbon - chemistry
Cell Membrane - chemistry
CHO Cells
Cricetinae
Cricetulus
Disruption
Energy transfer
Fluorescence
Fluorescence Resonance Energy Transfer - methods
Fretting
Imaging
Membranes
Microscopy, Fluorescence - methods
Nanoparticles
Phosphatidylcholines - analysis
Quantum Dots - chemistry
Surface-Active Agents - chemistry
X-Ray Diffraction
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Zusammenfassung:Newly-synthesized amphiphilic carbon dots were used for spectroscopic analysis and multicolour microscopic imaging of membranes and live cells. We show that Förster resonance energy transfer (FRET) occurred from the amphiphilic carbon dots to different membrane-associated fluorescence acceptors. The amphiphilic carbon dots enabled imaging of membrane disruption by the beta-amyloid peptide.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc03504f