Direct enzyme-substrate affinity determination by real-time hyperpolarized super(13)C-MRS

Direct enzyme-substrate affinity determination by real-time hyperpolarized super(13)C-MRS

A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D sub(4), 1- super(13)C]choline super(13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (K sub(m)), and inhibition. In a clinica...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2014-10, Vol.50 (89), p.13801-13804
Hauptverfasser: Friesen-Waldner, L J, Wiens, C N, Wade, T P, Thind, K, Sinclair, K P, Hovav, Y, Gomori, J M, Sosna, J, McKenzie, CA, Katz-Brull, R
Format: Artikel
Sprache:eng
Schlagworte:
Affinity
Choline
Inhibition
Metabolites
Oxidase
Real time
Scanners
Temporal resolution
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Zusammenfassung:A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D sub(4), 1- super(13)C]choline super(13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (K sub(m)), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 mu M were detected at a temporal resolution of 1 s.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc05418k