Development of codominant markers for identifying species hybrids

Herein we describe a simple method fordeveloping species-diagnostic markers thatwould permit the rapid identification of hybridindividuals. Our method relies on amplifiedlength polymorphism (AFLP) and single strandconformation polymorphism (SSCP) technologies,both of which can be performed in any molecularbiology facility using standard laboratoryequipment. We demonstrate the utility of theAFLP-SSCP method by developing threetaxon-specific markers that will be suitablefor monitoring introgression in endangeredKlamath basin suckers.[PUBLICATION ABSTRACT]