Partial Purification and Characterization of UDP-Glucose-4-Epimerase from Solieria chordalis (Rhodophyceae)

Partial Purification and Characterization of UDP-Glucose-4-Epimerase from Solieria chordalis (Rhodophyceae)

The partial purification of UDP-glucose-4-epimerase was carried out on the red macroalga Solieria chordalis. A 60-fold purification factor was obtained by chromatography on DEAE Protein-Pack 8 HR and Superose 12. Gel-filtration fast protein liquid chromatography (FPLC) showed that the enzyme had an...

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Veröffentlicht in:Botanica marina 2003-01, Vol.46 (1), p.107-111
Hauptverfasser: Goulard, F., Pondaven, P., Diouris, M., Deslandes, E., Floch, J. Y.
Format: Artikel
Sprache:eng
Schlagworte:
Marine
Rhodophyta
Solieria chordalis
UDP-glucose 4-epimerase
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Zusammenfassung:The partial purification of UDP-glucose-4-epimerase was carried out on the red macroalga Solieria chordalis. A 60-fold purification factor was obtained by chromatography on DEAE Protein-Pack 8 HR and Superose 12. Gel-filtration fast protein liquid chromatography (FPLC) showed that the enzyme had an apparent molecular mass of 130 kDa. The UDP-glucose-4-epimerase from Solieria chordalis does not require complementary NAD addition for activity, unlike the enzyme from some other organisms. Moreover, the optimal pH was at 8.5 and the apparent KM values for UDP-glucose and UDP-galactose were 87 μM and 630 μM, respectively.
ISSN:0006-8055
DOI:10.1515/BOT.2003.011