Birth of cloned mice from vaginal smear cells after somatic cell nuclear transfer
Less invasive methods for donor cell collection will facilitate reproduction of wild animals using somatic-cell nuclear transfer. Stages of the estrous cycle in mice have long been studies using somatic cells that can be collected from vaginal walls using cotton tipped swabs in a relatively non-inva...
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Veröffentlicht in: | Theriogenology 2017-05, Vol.94, p.79-85 |
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Zusammenfassung: | Less invasive methods for donor cell collection will facilitate reproduction of wild animals using somatic-cell nuclear transfer. Stages of the estrous cycle in mice have long been studies using somatic cells that can be collected from vaginal walls using cotton tipped swabs in a relatively non-invasive manner. In this study, we examined the feasibility of these cells as sources of nuclei for somatic-cell cloning using nuclear transfer. Estrous cycles generally comprise proestrus, estrus, metestrus, and diestrus stages. In the present experiments, more than 60% of cells were nucleated in vaginal smears from all but the estrus stage. However, after somatic-cell nuclear transfer of cells from proestrus, metestrus, and diestrus stages, 66%, 50%, and 72% of cloned embryos developed to the morula/blastocyst, and cloned female mouse birth rates after embryo transfer were 1.5%, 0.3%, and 1%, respectively. These results show that noninvasively collected vaginal smears contain somatic cells that can be used to clone female mice.
•Somatic cells in vaginal smear were used for somatic-cell cloning.•Through the estrous cycle, more than 60% nucleated cells exist in vaginal smear except at the estrus stage.•Birth of cloned mouse from these cells was demonstrated. |
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ISSN: | 0093-691X 1879-3231 |
DOI: | 10.1016/j.theriogenology.2017.02.012 |