Improved expression and immobilization of Geobacillus thermoleovorans CCR11 thermostable recombinant lipase

Production of recombinant thermo‐alkali‐stable lipase LipMatCCR11, expressed in Escherichia coli BL21 (DE3), was investigated via response surface methodology by using a face‐centered design with three levels of each factor. Additionally, improvement of the catalytic performance of expressed lipase...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2017-01, Vol.64 (1), p.62-69
Hauptverfasser:	Badillo‐Zeferino, Giselle‐Lilian, Ruiz‐López, Irving‐Israel, Oliart‐Ros, Rosamaŕıa, Sánchez‐Otero, María‐Guadalupe
Format:	Artikel
Sprache:	eng
Schlagworte:	Aroma Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Biochemistry Biotechnology E coli Enzymes, Immobilized - biosynthesis Enzymes, Immobilized - chemistry Enzymes, Immobilized - genetics Enzymes, Immobilized - isolation & purification Escherichia coli Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism esterification Esters Gene Expression Geobacillus - enzymology Geobacillus - genetics Geobacillus thermoleovorans CCR11 Immobilization Lipase Lipase - biosynthesis Lipase - chemistry Lipase - genetics Lipase - isolation & purification Polypropylene Recombinant recombinant lipase production Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Response surface methodology thermo‐alkali‐stable lipase Time measurement
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Zusammenfassung:	Production of recombinant thermo‐alkali‐stable lipase LipMatCCR11, expressed in Escherichia coli BL21 (DE3), was investigated via response surface methodology by using a face‐centered design with three levels of each factor. Additionally, improvement of the catalytic performance of expressed lipase was assessed by immobilization on microporous polypropylene. Results showed that inducer (isopropyl β‐d‐1‐thiogalactopyranoside [IPTG]) concentration and temperature were found to be the significant factors (P
ISSN:	0885-4513 1470-8744
DOI:	10.1002/bab.1444