A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging

Aggregation, red-NIR emission and light-up upon nucleic acid G-quadruplex binding have been investigated for a prototype core-extended naphthalene diimide, which is capable of fast cellular entry and nucleolar localization. Both high-level colocalization with an anti-G-quadruplex antibody and nucleo...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2017-02, Vol.53 (14), p.2268-2271
Hauptverfasser:	Doria, F, Nadai, M, Zuffo, M, Perrone, R, Freccero, M, Richter, S N
Format:	Artikel
Sprache:	eng
Schlagworte:	Agglomeration Antibodies Antibodies - chemistry Antibodies - immunology Binding Cell Nucleus - genetics Cell Nucleus - metabolism Chromosomal Proteins, Non-Histone - immunology Deoxyribonucleic acid Diimide Displacement Dyes Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism G-Quadruplexes HEK293 Cells Humans Imides - chemistry Imides - metabolism Microscopy, Confocal Naphthalenes - chemistry Naphthalenes - metabolism Spectrometry, Fluorescence
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Beschreibung
Zusammenfassung:	Aggregation, red-NIR emission and light-up upon nucleic acid G-quadruplex binding have been investigated for a prototype core-extended naphthalene diimide, which is capable of fast cellular entry and nucleolar localization. Both high-level colocalization with an anti-G-quadruplex antibody and nucleolin displacement reveal that the compound targets and thus makes visible nuclear DNA G-quadruplexes.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c6cc08492c