Vascular alterations after photodynamic therapy mediated by 5-aminolevulinic acid in oral leukoplakia

Impairment of vascular functions after photodynamic therapy (PDT) is frequently associated with tumor remission and is considered one of the main antineoplastic PDT effects. Vascular alterations in oral leukoplakia (OL) treated with PDT have not yet been described. The aim of this study was to evalu...

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Veröffentlicht in:Lasers in medical science 2017-02, Vol.32 (2), p.379-387
Hauptverfasser: Rosin, Flávia Cristina Perillo, Barcessat, Ana Rita Ribeiro, Borges, Giuliana Gadoni Giovanni, Ferreira, Luciana Gonçalves Valente, Corrêa, Luciana
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Sprache:eng
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Zusammenfassung:Impairment of vascular functions after photodynamic therapy (PDT) is frequently associated with tumor remission and is considered one of the main antineoplastic PDT effects. Vascular alterations in oral leukoplakia (OL) treated with PDT have not yet been described. The aim of this study was to evaluate the effect of topical 5-ALA-mediated PDT on the vascular network of 4NQO-induced OL in rats. After applying 4NQO topically on the tongue during 16 weeks, there was induction of dysplastic lesions, which were treated with two PDT sessions (with an interval of 72 h between them), using topical application of 5-ALA and posterior irradiation with a laser (90 J/cm 2 fluency). Histological sections of the tongues were obtained and analyzed concerning plasmatic exudation and microvessel density after immunolabeling with CD31 and CD34 vessel markers. There was intense plasmatic exudation after 6 h of the first PDT session; at 6 h of the second PDT session, there was a significant reduction in the density of CD31- and CD34-positive microvessels in comparison to controls ( p  
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-016-2127-0