Redox status of serum apolipoprotein E and its impact on HDL cholesterol levels
Apolipoprotein E (apoE) is closely involved in the pathogenesis of apoE-related diseases, such as Alzheimer's disease and cardiovascular disease. The redox modulation of cysteine-thiols in a protein is involved in various pathophysiological regulations; however, that of apoE has not been studie...
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Veröffentlicht in: | Clinical biochemistry 2017-09, Vol.50 (13-14), p.777-783 |
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Sprache: | eng |
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Zusammenfassung: | Apolipoprotein E (apoE) is closely involved in the pathogenesis of apoE-related diseases, such as Alzheimer's disease and cardiovascular disease. The redox modulation of cysteine-thiols in a protein is involved in various pathophysiological regulations; however, that of apoE has not been studied in detail. Herein, we devised an analytical method to determine the redox status of serum apoE and assessed its relation to serum cholesterol levels and apoE phenotype.
The present method was based on a band shift assay, using a photocleavable maleimide-conjugated polyethylene glycol.
The basic characteristics of the present method were found to be satisfactory to determine the redox status of serum apoE quantitatively. Serum apoE was separated into its reduced-form (r-), non-reduced-form (nr-), apoE-AII complex, and homodimer using this method. R-apoE could be detected as a 40-kDa band, whereas nr-apoE remained as monomeric apoE. R-apoE displayed a preference for VLDL; however, the levels showed the correlation with HDL-cholesterol levels (p |
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ISSN: | 0009-9120 1873-2933 |
DOI: | 10.1016/j.clinbiochem.2017.03.021 |