Sequencing of the Hepatitis D Virus RNA WHO International Standard

Highlights • The WHO Standard for HDV RNA was sequenced by Sanger sequencing and RNA-seq. • There was 100% agreement between Sanger and RNA-seq consensus sequences. • The consensus sequence had 89.0% identity to the published WHO sequence. • The best matching sequence (99.2%) was from the same publi...

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Veröffentlicht in:	Journal of clinical virology 2017-05, Vol.90, p.52-56
Hauptverfasser:	Pyne, M.T, Mallory, M.A, Xie, H.B, Mei, Y, Schlaberg, R, Hillyard, D.R
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Calibration Genome, Viral HDV Hepatitis D Virus Hepatitis Delta Virus - genetics Humans Infectious Disease Reference Standards RNA, Viral - genetics Sequence Analysis, DNA Sequencing Viral hepatitis WHO International Standard
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Beschreibung
Zusammenfassung:	Highlights • The WHO Standard for HDV RNA was sequenced by Sanger sequencing and RNA-seq. • There was 100% agreement between Sanger and RNA-seq consensus sequences. • The consensus sequence had 89.0% identity to the published WHO sequence. • The best matching sequence (99.2%) was from the same published study. • It appears that the identifier and sequence have been incorrectly associated.
ISSN:	1386-6532 1873-5967
DOI:	10.1016/j.jcv.2017.03.009