NDRG2 Inhibits Migration of Breast Cancer Cells Through Upregulating PTEN Expression

Objective To investigate the effects of N-Myc downstream regulated gene 2 (NDRG2) on the migration of human breast cancer cells by using two parallel cell lines MCF-7 and LM-MCF with different metastatic abilities. Methods The expression level of NDRG2 and PTEN in breast cancer cells were detected by RT-PCR and Western blot. The effects of overexpressed or down-regulated NDRG2 on the migration of breast cancer cells were investigated by Transwell migration test. The regulation mechanism between NDRG2 and PTEN on cell migration was detected via co-transfecting breast cancer cells with pCMV-NDRG2 and PTEN siRNA (or NDRG2 siRNA and pCMV-PTEN). Results The mRNA and protein expression levels of NDRG2 and PTEN in MCF-7 cells were higher than those in LM-MCF-7/MDA-MB-231 cells(P