CRISPR-Cas system presents multiple transcriptional units including antisense RNAs that are expressed in minimal medium and upregulated by pH in Salmonella enterica serovar Typhi

The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in mi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Microbiology (Society for General Microbiology) 2017-02, Vol.163 (2), p.253-265
Hauptverfasser: Medina-Aparicio, Liliana, Rebollar-Flores, Javier E, Beltrán-Luviano, América A, Vázquez, Alejandra, Gutiérrez-Ríos, Rosa M, Olvera, Leticia, Calva, Edmundo, Hernández-Lucas, Ismael
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 265
container_issue 2
container_start_page 253
container_title Microbiology (Society for General Microbiology)
container_volume 163
creator Medina-Aparicio, Liliana
Rebollar-Flores, Javier E
Beltrán-Luviano, América A
Vázquez, Alejandra
Gutiérrez-Ríos, Rosa M
Olvera, Leticia
Calva, Edmundo
Hernández-Lucas, Ismael
description The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in minimal medium and are up-regulated by pH. In addition, a transcriptional characterization of cas3 and ascse2-1 is included herein.
doi_str_mv 10.1099/mic.0.000414
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1881755756</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1881755756</sourcerecordid><originalsourceid>FETCH-LOGICAL-c362t-c05e527a6040cff799748015278e5d966920fb74e76301acc369ebff40cefe3d3</originalsourceid><addsrcrecordid>eNqNkU9v1DAQxSMEoqVw44x85ECWcRzHybFaAa1UAdqWc-R1Jq2R7QT_QezX4hMyqy2cOXnk93tPo3lV9ZrDhsMwvPfWbGADAC1vn1TnvO1k3UAPT2kWEmroVXNWvUjpOwCJwJ9XZ03fKGhUe1793u6ub7_u6q1OLB1SRs_WiAlDTswXl-3qkOWoQzLRrtkuQTtWgiXZBuPKZMM90yFbsiRku8-XieUHnZmOyPDXMSvhRCzzNlhPZo-TLZ48Eysk3xenMxH7A1uvjtytdn4J6JxmtAVGazRLGJefOrK7w_pgX1bPZu0Svnp8L6pvHz_cba_qmy-frreXN7URXZNrAxJlo3QHLZh5VsOg2h44ffUop6HrhgbmvWpRdQK4NuQacD_PROOMYhIX1dtT7hqXHwVTHr1N5rhYwKWkkfc9V1Iq2f0HqmQLQghJ6LsTauKSUsR5XCPdJR5GDuOxULKaEcZToYS_eUwue7rcP_hvg-IPTJSfNw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1875403335</pqid></control><display><type>article</type><title>CRISPR-Cas system presents multiple transcriptional units including antisense RNAs that are expressed in minimal medium and upregulated by pH in Salmonella enterica serovar Typhi</title><source>MEDLINE</source><source>PubMed Central</source><creator>Medina-Aparicio, Liliana ; Rebollar-Flores, Javier E ; Beltrán-Luviano, América A ; Vázquez, Alejandra ; Gutiérrez-Ríos, Rosa M ; Olvera, Leticia ; Calva, Edmundo ; Hernández-Lucas, Ismael</creator><creatorcontrib>Medina-Aparicio, Liliana ; Rebollar-Flores, Javier E ; Beltrán-Luviano, América A ; Vázquez, Alejandra ; Gutiérrez-Ríos, Rosa M ; Olvera, Leticia ; Calva, Edmundo ; Hernández-Lucas, Ismael</creatorcontrib><description>The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in minimal medium and are up-regulated by pH. In addition, a transcriptional characterization of cas3 and ascse2-1 is included herein.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/mic.0.000414</identifier><identifier>PMID: 28270274</identifier><language>eng</language><publisher>England</publisher><subject>Chloramphenicol O-Acetyltransferase - metabolism ; CRISPR-Associated Proteins - genetics ; CRISPR-Cas Systems - genetics ; DNA Helicases - genetics ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Bacterial - genetics ; RNA, Antisense - genetics ; Salmonella enterica ; Salmonella typhi - genetics ; Transcription, Genetic - genetics ; Transcriptional Activation - genetics ; Up-Regulation - genetics</subject><ispartof>Microbiology (Society for General Microbiology), 2017-02, Vol.163 (2), p.253-265</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-c05e527a6040cff799748015278e5d966920fb74e76301acc369ebff40cefe3d3</citedby><cites>FETCH-LOGICAL-c362t-c05e527a6040cff799748015278e5d966920fb74e76301acc369ebff40cefe3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28270274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Medina-Aparicio, Liliana</creatorcontrib><creatorcontrib>Rebollar-Flores, Javier E</creatorcontrib><creatorcontrib>Beltrán-Luviano, América A</creatorcontrib><creatorcontrib>Vázquez, Alejandra</creatorcontrib><creatorcontrib>Gutiérrez-Ríos, Rosa M</creatorcontrib><creatorcontrib>Olvera, Leticia</creatorcontrib><creatorcontrib>Calva, Edmundo</creatorcontrib><creatorcontrib>Hernández-Lucas, Ismael</creatorcontrib><title>CRISPR-Cas system presents multiple transcriptional units including antisense RNAs that are expressed in minimal medium and upregulated by pH in Salmonella enterica serovar Typhi</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in minimal medium and are up-regulated by pH. In addition, a transcriptional characterization of cas3 and ascse2-1 is included herein.</description><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>CRISPR-Associated Proteins - genetics</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>DNA Helicases - genetics</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Gene Expression Regulation, Bacterial - genetics</subject><subject>RNA, Antisense - genetics</subject><subject>Salmonella enterica</subject><subject>Salmonella typhi - genetics</subject><subject>Transcription, Genetic - genetics</subject><subject>Transcriptional Activation - genetics</subject><subject>Up-Regulation - genetics</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxSMEoqVw44x85ECWcRzHybFaAa1UAdqWc-R1Jq2R7QT_QezX4hMyqy2cOXnk93tPo3lV9ZrDhsMwvPfWbGADAC1vn1TnvO1k3UAPT2kWEmroVXNWvUjpOwCJwJ9XZ03fKGhUe1793u6ub7_u6q1OLB1SRs_WiAlDTswXl-3qkOWoQzLRrtkuQTtWgiXZBuPKZMM90yFbsiRku8-XieUHnZmOyPDXMSvhRCzzNlhPZo-TLZ48Eysk3xenMxH7A1uvjtytdn4J6JxmtAVGazRLGJefOrK7w_pgX1bPZu0Svnp8L6pvHz_cba_qmy-frreXN7URXZNrAxJlo3QHLZh5VsOg2h44ffUop6HrhgbmvWpRdQK4NuQacD_PROOMYhIX1dtT7hqXHwVTHr1N5rhYwKWkkfc9V1Iq2f0HqmQLQghJ6LsTauKSUsR5XCPdJR5GDuOxULKaEcZToYS_eUwue7rcP_hvg-IPTJSfNw</recordid><startdate>201702</startdate><enddate>201702</enddate><creator>Medina-Aparicio, Liliana</creator><creator>Rebollar-Flores, Javier E</creator><creator>Beltrán-Luviano, América A</creator><creator>Vázquez, Alejandra</creator><creator>Gutiérrez-Ríos, Rosa M</creator><creator>Olvera, Leticia</creator><creator>Calva, Edmundo</creator><creator>Hernández-Lucas, Ismael</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>201702</creationdate><title>CRISPR-Cas system presents multiple transcriptional units including antisense RNAs that are expressed in minimal medium and upregulated by pH in Salmonella enterica serovar Typhi</title><author>Medina-Aparicio, Liliana ; Rebollar-Flores, Javier E ; Beltrán-Luviano, América A ; Vázquez, Alejandra ; Gutiérrez-Ríos, Rosa M ; Olvera, Leticia ; Calva, Edmundo ; Hernández-Lucas, Ismael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-c05e527a6040cff799748015278e5d966920fb74e76301acc369ebff40cefe3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>CRISPR-Associated Proteins - genetics</topic><topic>CRISPR-Cas Systems - genetics</topic><topic>DNA Helicases - genetics</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Gene Expression Regulation, Bacterial - genetics</topic><topic>RNA, Antisense - genetics</topic><topic>Salmonella enterica</topic><topic>Salmonella typhi - genetics</topic><topic>Transcription, Genetic - genetics</topic><topic>Transcriptional Activation - genetics</topic><topic>Up-Regulation - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Medina-Aparicio, Liliana</creatorcontrib><creatorcontrib>Rebollar-Flores, Javier E</creatorcontrib><creatorcontrib>Beltrán-Luviano, América A</creatorcontrib><creatorcontrib>Vázquez, Alejandra</creatorcontrib><creatorcontrib>Gutiérrez-Ríos, Rosa M</creatorcontrib><creatorcontrib>Olvera, Leticia</creatorcontrib><creatorcontrib>Calva, Edmundo</creatorcontrib><creatorcontrib>Hernández-Lucas, Ismael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Medina-Aparicio, Liliana</au><au>Rebollar-Flores, Javier E</au><au>Beltrán-Luviano, América A</au><au>Vázquez, Alejandra</au><au>Gutiérrez-Ríos, Rosa M</au><au>Olvera, Leticia</au><au>Calva, Edmundo</au><au>Hernández-Lucas, Ismael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CRISPR-Cas system presents multiple transcriptional units including antisense RNAs that are expressed in minimal medium and upregulated by pH in Salmonella enterica serovar Typhi</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2017-02</date><risdate>2017</risdate><volume>163</volume><issue>2</issue><spage>253</spage><epage>265</epage><pages>253-265</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in minimal medium and are up-regulated by pH. In addition, a transcriptional characterization of cas3 and ascse2-1 is included herein.</abstract><cop>England</cop><pmid>28270274</pmid><doi>10.1099/mic.0.000414</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1350-0872
ispartof Microbiology (Society for General Microbiology), 2017-02, Vol.163 (2), p.253-265
issn 1350-0872
1465-2080
language eng
recordid cdi_proquest_miscellaneous_1881755756
source MEDLINE; PubMed Central
subjects Chloramphenicol O-Acetyltransferase - metabolism
CRISPR-Associated Proteins - genetics
CRISPR-Cas Systems - genetics
DNA Helicases - genetics
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Bacterial - genetics
RNA, Antisense - genetics
Salmonella enterica
Salmonella typhi - genetics
Transcription, Genetic - genetics
Transcriptional Activation - genetics
Up-Regulation - genetics
title CRISPR-Cas system presents multiple transcriptional units including antisense RNAs that are expressed in minimal medium and upregulated by pH in Salmonella enterica serovar Typhi
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T11%3A47%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CRISPR-Cas%20system%20presents%20multiple%20transcriptional%20units%20including%20antisense%20RNAs%20that%20are%20expressed%20in%20minimal%20medium%20and%20upregulated%20by%20pH%20in%20Salmonella%20enterica%20serovar%20Typhi&rft.jtitle=Microbiology%20(Society%20for%20General%20Microbiology)&rft.au=Medina-Aparicio,%20Liliana&rft.date=2017-02&rft.volume=163&rft.issue=2&rft.spage=253&rft.epage=265&rft.pages=253-265&rft.issn=1350-0872&rft.eissn=1465-2080&rft_id=info:doi/10.1099/mic.0.000414&rft_dat=%3Cproquest_cross%3E1881755756%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1875403335&rft_id=info:pmid/28270274&rfr_iscdi=true