CRISPR-Cas system presents multiple transcriptional units including antisense RNAs that are expressed in minimal medium and upregulated by pH in Salmonella enterica serovar Typhi

The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in mi...

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Veröffentlicht in:	Microbiology (Society for General Microbiology) 2017-02, Vol.163 (2), p.253-265
Hauptverfasser:	Medina-Aparicio, Liliana, Rebollar-Flores, Javier E, Beltrán-Luviano, América A, Vázquez, Alejandra, Gutiérrez-Ríos, Rosa M, Olvera, Leticia, Calva, Edmundo, Hernández-Lucas, Ismael
Format:	Artikel
Sprache:	eng
Schlagworte:	Chloramphenicol O-Acetyltransferase - metabolism CRISPR-Associated Proteins - genetics CRISPR-Cas Systems - genetics DNA Helicases - genetics Electrophoretic Mobility Shift Assay Gene Expression Regulation, Bacterial - genetics RNA, Antisense - genetics Salmonella enterica Salmonella typhi - genetics Transcription, Genetic - genetics Transcriptional Activation - genetics Up-Regulation - genetics
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Zusammenfassung:	The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in minimal medium and are up-regulated by pH. In addition, a transcriptional characterization of cas3 and ascse2-1 is included herein.
ISSN:	1350-0872 1465-2080
DOI:	10.1099/mic.0.000414