Molecular Characterization of Anaplasma sp. in Free-Living Gray Brockets (Mazama gouazoubira)
Bacteria in the genus Anaplasma are responsible for diseases in animals and humans. Studies carried out in Brazil have demonstrated that Brazilian deer are able to act as hosts of agents in the family Anaplasmataceae and are possibly potential reservoirs of these pathogens. Molecular and phylogeneti...
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Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2017-03, Vol.17 (3), p.165-171 |
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Zusammenfassung: | Bacteria in the genus
Anaplasma
are responsible for diseases in animals and humans. Studies carried out in Brazil have demonstrated that Brazilian deer are able to act as hosts of agents in the family Anaplasmataceae and are possibly potential reservoirs of these pathogens. Molecular and phylogenetic studies have been carried out on samples of two gray brocket specimens (
Mazama gouazoubira
) from the city of Guarapuava, state of Paraná, Brazil, for the detection of
Anaplasma
sp. in these animals. Partial nucleotide sequences of the genes
16S rRNA
and
groESL
were used for phylogenetic analyses and compared with other 13 and 17 partial sequences of the respective genes obtained in
GenBank
. These assessments showed topological incongruence among the trees generated in the phylogenetic analyses. Phylogenetic analysis based on the gene 16S
rRNA
of the genotypes amplified in the samples of this study was similar to those of
A. bovis
detected in dogs and wild deer in Japan, whereas studies carried out on gene
groESL
indicated proximity with sequences of
Anaplasma
sp. that were also isolated in deer in Japan and allocated in the same clade of partial sequences of
A. phagocytophilum.
As the 16S
rRNA
gene is highly conserved, with few polymorphic positions, it may show low reliability for studies on phylogenetic positioning. The present study detected an
Anaplasma
sp. genotype in two specimens of
M. gouazoubira
in southern Brazil, which may mean that this agent possibly circulates in deer populations, and demonstrated the need for studies related to the possible role of deer in enzootic cycles of Anaplasmataceae in Brazil. |
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ISSN: | 1530-3667 1557-7759 |
DOI: | 10.1089/vbz.2016.2026 |