Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells

Epithelial-mesenchymal transition (EMT) is a process in tumor progression during which cancer cells undergo dramatic changes acquiring highly invasive properties. In a widespread adoption of TGF-β-induced EMT model, we have previously observed that expression of bisecting GlcNAc on N -glycans was dr...

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Veröffentlicht in:Glycoconjugate journal 2017-04, Vol.34 (2), p.219-227
Hauptverfasser: Xu, Qingsong, Niu, Xueming, Wang, Wenjing, Yang, Wen, Du, Yuguang, Gu, Jianguo, Song, Linsheng
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Sprache:eng
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Zusammenfassung:Epithelial-mesenchymal transition (EMT) is a process in tumor progression during which cancer cells undergo dramatic changes acquiring highly invasive properties. In a widespread adoption of TGF-β-induced EMT model, we have previously observed that expression of bisecting GlcNAc on N -glycans was dramatically decreased. Herein, we performed in vitro studies with the MCF10A cell line. In response to low cell density, MCF10A cells suffered spontaneously morphologic and phenotypic EMT-like changes, including elongated spindle shape, extended out from edge of the cell sheet, cytoskeleton reorganization, vimentin and fibronectin up-regulation, catenins redistribution, and cadherin switching. Moreover, these phenotypic changes were associated with specific N -glycan alterations. Interestingly, the amounts of bisecting GlcNAc structure were declined, by contrast, the formation of β1-6 GlcNAc branches were obviously up-regulated during the EMT induced by sparse cell conditions. We further investigated N -glycans on the β1-integrin, which is a good target of some glycosyltransferases. The reactivity with E4-PHA lectin decreased, whereas the staining for L4-PHA lectin, which recognizes branched GlcNAc, increased in sparse cell conditions compared with dense cell conditions. Taken together, these results demonstrated that specific N -glycan alterations are coupled in EMT process and promoted cells migration at a low cell density.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-016-9754-3