Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide
Aim To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC‐23 cells. It was hypothesized that MDPC‐23 odontoblast‐like cells may function as odontoclasts under the influence of LPS. Methodology MDPC‐23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LP...
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| Veröffentlicht in: | International endodontic journal 2018-02, Vol.51 (S2), p.e115-e124 |
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| creator | Li, C. Qi, W. T. Jiang, H. W. |
| description | Aim
To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC‐23 cells. It was hypothesized that MDPC‐23 odontoblast‐like cells may function as odontoclasts under the influence of LPS.
Methodology
MDPC‐23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast‐like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's t‐test and one‐way anova were used in statistical analysis.
Results
TRAP‐positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg mL−1 LPS‐induced cells (P |
| doi_str_mv | 10.1111/iej.12771 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1880469328</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1880469328</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4141-b3e1903ed25ee03bee46f2c334b28f378dd61b1f7ead229b3d8569c4c0f952c83</originalsourceid><addsrcrecordid>eNp1kM1KAzEUhYMotlYXvoAMuNHF1PzMT2YptWpFqQvdCSGT3NGUdDJOOkp3PoLP6JOYWnUheDaXe_k4nHsQ2id4SIJODMyGhOY52UB9wrI0pmlBNlEfk4TFlPO0h3a8n2GMU8zINupRzoLypI8eptrVC6es9Av3CDV44yNXRXPXeYga2Rhr5cfbu4bWvICObs5uR2GlLFJgrY9MrTsV7uUysqZxjbNLL5V6kq3RsIu2Kmk97H3PAbo_H9-NLuPr6cVkdHodq4QkJC4ZkAIz0DQFwKwESLKKKsaSkvKK5VzrjJSkykFqSouSaZ5mhUoUroqUKs4G6Gjt27TuuQO_EHPjV_lkDeEPQTjHSVYwukIP_6Az17V1SCcoxgQzigsWqOM1pVrnfQuVaFozl-1SECxWlYtQufiqPLAH345dOQf9S_50HICTNfBqLCz_dxKT8dXa8hMQ_4wl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2001032093</pqid></control><display><type>article</type><title>Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Li, C. ; Qi, W. T. ; Jiang, H. W.</creator><creatorcontrib>Li, C. ; Qi, W. T. ; Jiang, H. W.</creatorcontrib><description>Aim
To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC‐23 cells. It was hypothesized that MDPC‐23 odontoblast‐like cells may function as odontoclasts under the influence of LPS.
Methodology
MDPC‐23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast‐like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's t‐test and one‐way anova were used in statistical analysis.
Results
TRAP‐positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg mL−1 LPS‐induced cells (P < 0.05). Osteoclast‐specific proteins such as TRAP cathepsin K and Rac1 were upregulated in the 1 μg mL−1 LPS‐treated cells (P < 0.05), whilst the expression of marker proteins of the RANKL‐RANK signalling pathway (RANKL, RANK and TRAF6) in the induced cells was not significantly changed (P > 0.05). ROS production was observed in the 1 μg mL−1 LPS treatment group (P < 0.05), but no significant differences were observed in the level of RANKL in the cell supernatant between the LPS‐treated group and the control group (P > 0.05).
Conclusions
A known value of 1 μg mL−1 LPS might induce odontoblast‐like MDPC‐23 cells to generate odontoclast‐like cells or to function as odontoclasts. The data might provide a new explanation for the precursors of odontoclasts and root resorption.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.12771</identifier><identifier>PMID: 28333374</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Blotting, Western ; Cathepsin K ; Cell Differentiation - drug effects ; Cell Line ; Cell Proliferation - drug effects ; Dental Papilla - cytology ; Dental Papilla - drug effects ; Dental Papilla - metabolism ; Dentistry ; Endodontics ; Enzyme-Linked Immunosorbent Assay ; lipopolysaccharide ; Lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Mice ; Microscopy, Confocal ; odontoclast ; Odontoclasts ; Odontogenesis - drug effects ; osteoclast ; Osteoclasts - drug effects ; Osteoclasts - metabolism ; Rac1 protein ; RANKL‐RANK signalling pathway ; Reactive Oxygen Species - metabolism ; Rodents ; Root resorption ; Signal transduction ; Statistical analysis ; TRAF6 protein ; TRANCE protein ; Western blotting</subject><ispartof>International endodontic journal, 2018-02, Vol.51 (S2), p.e115-e124</ispartof><rights>2017 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2017 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2018 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4141-b3e1903ed25ee03bee46f2c334b28f378dd61b1f7ead229b3d8569c4c0f952c83</citedby><cites>FETCH-LOGICAL-c4141-b3e1903ed25ee03bee46f2c334b28f378dd61b1f7ead229b3d8569c4c0f952c83</cites><orcidid>0000-0002-2351-9986</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.12771$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.12771$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28333374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, C.</creatorcontrib><creatorcontrib>Qi, W. T.</creatorcontrib><creatorcontrib>Jiang, H. W.</creatorcontrib><title>Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC‐23 cells. It was hypothesized that MDPC‐23 odontoblast‐like cells may function as odontoclasts under the influence of LPS.
Methodology
MDPC‐23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast‐like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's t‐test and one‐way anova were used in statistical analysis.
Results
TRAP‐positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg mL−1 LPS‐induced cells (P < 0.05). Osteoclast‐specific proteins such as TRAP cathepsin K and Rac1 were upregulated in the 1 μg mL−1 LPS‐treated cells (P < 0.05), whilst the expression of marker proteins of the RANKL‐RANK signalling pathway (RANKL, RANK and TRAF6) in the induced cells was not significantly changed (P > 0.05). ROS production was observed in the 1 μg mL−1 LPS treatment group (P < 0.05), but no significant differences were observed in the level of RANKL in the cell supernatant between the LPS‐treated group and the control group (P > 0.05).
Conclusions
A known value of 1 μg mL−1 LPS might induce odontoblast‐like MDPC‐23 cells to generate odontoclast‐like cells or to function as odontoclasts. The data might provide a new explanation for the precursors of odontoclasts and root resorption.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cathepsin K</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Dental Papilla - cytology</subject><subject>Dental Papilla - drug effects</subject><subject>Dental Papilla - metabolism</subject><subject>Dentistry</subject><subject>Endodontics</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>odontoclast</subject><subject>Odontoclasts</subject><subject>Odontogenesis - drug effects</subject><subject>osteoclast</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoclasts - metabolism</subject><subject>Rac1 protein</subject><subject>RANKL‐RANK signalling pathway</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rodents</subject><subject>Root resorption</subject><subject>Signal transduction</subject><subject>Statistical analysis</subject><subject>TRAF6 protein</subject><subject>TRANCE protein</subject><subject>Western blotting</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1KAzEUhYMotlYXvoAMuNHF1PzMT2YptWpFqQvdCSGT3NGUdDJOOkp3PoLP6JOYWnUheDaXe_k4nHsQ2id4SIJODMyGhOY52UB9wrI0pmlBNlEfk4TFlPO0h3a8n2GMU8zINupRzoLypI8eptrVC6es9Av3CDV44yNXRXPXeYga2Rhr5cfbu4bWvICObs5uR2GlLFJgrY9MrTsV7uUysqZxjbNLL5V6kq3RsIu2Kmk97H3PAbo_H9-NLuPr6cVkdHodq4QkJC4ZkAIz0DQFwKwESLKKKsaSkvKK5VzrjJSkykFqSouSaZ5mhUoUroqUKs4G6Gjt27TuuQO_EHPjV_lkDeEPQTjHSVYwukIP_6Az17V1SCcoxgQzigsWqOM1pVrnfQuVaFozl-1SECxWlYtQufiqPLAH345dOQf9S_50HICTNfBqLCz_dxKT8dXa8hMQ_4wl</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Li, C.</creator><creator>Qi, W. T.</creator><creator>Jiang, H. W.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2351-9986</orcidid></search><sort><creationdate>201802</creationdate><title>Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide</title><author>Li, C. ; Qi, W. T. ; Jiang, H. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4141-b3e1903ed25ee03bee46f2c334b28f378dd61b1f7ead229b3d8569c4c0f952c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cathepsin K</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Dental Papilla - cytology</topic><topic>Dental Papilla - drug effects</topic><topic>Dental Papilla - metabolism</topic><topic>Dentistry</topic><topic>Endodontics</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>odontoclast</topic><topic>Odontoclasts</topic><topic>Odontogenesis - drug effects</topic><topic>osteoclast</topic><topic>Osteoclasts - drug effects</topic><topic>Osteoclasts - metabolism</topic><topic>Rac1 protein</topic><topic>RANKL‐RANK signalling pathway</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rodents</topic><topic>Root resorption</topic><topic>Signal transduction</topic><topic>Statistical analysis</topic><topic>TRAF6 protein</topic><topic>TRANCE protein</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, C.</creatorcontrib><creatorcontrib>Qi, W. T.</creatorcontrib><creatorcontrib>Jiang, H. W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, C.</au><au>Qi, W. T.</au><au>Jiang, H. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2018-02</date><risdate>2018</risdate><volume>51</volume><issue>S2</issue><spage>e115</spage><epage>e124</epage><pages>e115-e124</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC‐23 cells. It was hypothesized that MDPC‐23 odontoblast‐like cells may function as odontoclasts under the influence of LPS.
Methodology
MDPC‐23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast‐like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's t‐test and one‐way anova were used in statistical analysis.
Results
TRAP‐positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg mL−1 LPS‐induced cells (P < 0.05). Osteoclast‐specific proteins such as TRAP cathepsin K and Rac1 were upregulated in the 1 μg mL−1 LPS‐treated cells (P < 0.05), whilst the expression of marker proteins of the RANKL‐RANK signalling pathway (RANKL, RANK and TRAF6) in the induced cells was not significantly changed (P > 0.05). ROS production was observed in the 1 μg mL−1 LPS treatment group (P < 0.05), but no significant differences were observed in the level of RANKL in the cell supernatant between the LPS‐treated group and the control group (P > 0.05).
Conclusions
A known value of 1 μg mL−1 LPS might induce odontoblast‐like MDPC‐23 cells to generate odontoclast‐like cells or to function as odontoclasts. The data might provide a new explanation for the precursors of odontoclasts and root resorption.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28333374</pmid><doi>10.1111/iej.12771</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-2351-9986</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Blotting, Western Cathepsin K Cell Differentiation - drug effects Cell Line Cell Proliferation - drug effects Dental Papilla - cytology Dental Papilla - drug effects Dental Papilla - metabolism Dentistry Endodontics Enzyme-Linked Immunosorbent Assay lipopolysaccharide Lipopolysaccharides Lipopolysaccharides - pharmacology Mice Microscopy, Confocal odontoclast Odontoclasts Odontogenesis - drug effects osteoclast Osteoclasts - drug effects Osteoclasts - metabolism Rac1 protein RANKL‐RANK signalling pathway Reactive Oxygen Species - metabolism Rodents Root resorption Signal transduction Statistical analysis TRAF6 protein TRANCE protein Western blotting |
| title | Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide |
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