Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide

Aim To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC‐23 cells. It was hypothesized that MDPC‐23 odontoblast‐like cells may function as odontoclasts under the influence of LPS. Methodology MDPC‐23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast‐like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's t‐test and one‐way anova were used in statistical analysis. Results TRAP‐positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg mL−1 LPS‐induced cells (P