Unnatural amino acid mutagenesis in mapping ion channel function

Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure–function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing ex...

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Veröffentlicht in:	Current opinion in neurobiology 2003-06, Vol.13 (3), p.264-270
Hauptverfasser:	Beene, Darren L, Dougherty, Dennis A, Lester, Henry A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - chemistry Amino Acids - physiology Animals Chromosome Mapping - methods Humans Ion Channels - chemistry Ion Channels - physiology Mutagenesis, Site-Directed - physiology Oocytes Structure-Activity Relationship Xenopus
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure–function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.
ISSN:	0959-4388 1873-6882
DOI:	10.1016/S0959-4388(03)00068-0