A colorimetric and fluorescent pH probe for imaging in E. coli cells

A colorimetric and fluorescent pH probe for imaging in E. coli cells

A colorimetric and fluorescent pH probe 1-(4-methylphenyl)-3-(4-dimethylaminophenyl) acrylketone ( MDAK ) was facilely prepared. The probe exhibited strong pH-dependent behavior and responded linearly and rapidly to minor pH fluctuations within the range of 1.53–3.96. In addition, MDAK displayed a n...

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Veröffentlicht in:RSC advances 2017, Vol.7 (2), p.964-970
Hauptverfasser: Chao, Jianbin, Song, Kailun, Wang, Huijuan, Li, Zhiqing, Zhang, Yongbin, Yin, Caixia, Huo, Fangjun, Wang, Juanjuan, Zhang, Ting
Format: Artikel
Sprache:eng
Schlagworte:
Colorimetry
Fluctuation
Fluid flow
Fluorescence
Imaging
Metal ions
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Beschreibung
Zusammenfassung:A colorimetric and fluorescent pH probe 1-(4-methylphenyl)-3-(4-dimethylaminophenyl) acrylketone ( MDAK ) was facilely prepared. The probe exhibited strong pH-dependent behavior and responded linearly and rapidly to minor pH fluctuations within the range of 1.53–3.96. In addition, MDAK displayed a notably large Stokes shift of 121 nm. MDAK possessed a highly selective response to H + over other metal ions, amion acids and displayed good photostability and excellent reversibility. Furthermore, it can be applied to visualize extreme acidity in E. coli cells.
ISSN:2046-2069
2046-2069
DOI:10.1039/C6RA24885C