Cloning, expression and functional characterization on vitellogenesis of estrogen receptors in Scatophagus argus

Highlights • erα, erβ1 and erβ2 partial cDNA of S. argus were cloned. • vtg-A, vtg-B and vtg-C partial cDNA of S. argus were cloned. • Ontogeny expression of three ers and three vtgs in female livers were performed. • The contribution of each Er to the regulation of different vtgs were determined....

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Veröffentlicht in:General and comparative endocrinology 2017-05, Vol.246, p.37-45
Hauptverfasser: Cui, Xue-Fan, Zhao, Yuan, Chen, Hua-Pu, Deng, Si-Ping, Jiang, Dong-Neng, Wu, Tian-Li, Zhu, Chun-Hua, Li, Guang-Li
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container_end_page 45
container_issue
container_start_page 37
container_title General and comparative endocrinology
container_volume 246
creator Cui, Xue-Fan
Zhao, Yuan
Chen, Hua-Pu
Deng, Si-Ping
Jiang, Dong-Neng
Wu, Tian-Li
Zhu, Chun-Hua
Li, Guang-Li
description Highlights • erα, erβ1 and erβ2 partial cDNA of S. argus were cloned. • vtg-A, vtg-B and vtg-C partial cDNA of S. argus were cloned. • Ontogeny expression of three ers and three vtgs in female livers were performed. • The contribution of each Er to the regulation of different vtgs were determined.
doi_str_mv 10.1016/j.ygcen.2017.03.002
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subjects Amino Acid Sequence
Animals
Cloning, Molecular
Endocrinology & Metabolism
Enzyme-Linked Immunosorbent Assay
Estrogen
Estrogen receptor
Estrogen Receptor alpha - genetics
Estrogen Receptor alpha - metabolism
Estrogen receptor antagonist
Estrogen Receptor beta - genetics
Estrogen Receptor beta - metabolism
Female
Liver - metabolism
Male
Perciformes - genetics
Perciformes - growth & development
Perciformes - metabolism
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Scatophagus argus
Sequence Homology, Amino Acid
Vitellogenesis - physiology
Vitellogenin
Vitellogenins - genetics
Vitellogenins - metabolism
title Cloning, expression and functional characterization on vitellogenesis of estrogen receptors in Scatophagus argus
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