Cloning, expression and functional characterization on vitellogenesis of estrogen receptors in Scatophagus argus

Highlights • erα, erβ1 and erβ2 partial cDNA of S. argus were cloned. • vtg-A, vtg-B and vtg-C partial cDNA of S. argus were cloned. • Ontogeny expression of three ers and three vtgs in female livers were performed. • The contribution of each Er to the regulation of different vtgs were determined....

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Veröffentlicht in:	General and comparative endocrinology 2017-05, Vol.246, p.37-45
Hauptverfasser:	Cui, Xue-Fan, Zhao, Yuan, Chen, Hua-Pu, Deng, Si-Ping, Jiang, Dong-Neng, Wu, Tian-Li, Zhu, Chun-Hua, Li, Guang-Li
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Cloning, Molecular Endocrinology & Metabolism Enzyme-Linked Immunosorbent Assay Estrogen Estrogen receptor Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism Estrogen receptor antagonist Estrogen Receptor beta - genetics Estrogen Receptor beta - metabolism Female Liver - metabolism Male Perciformes - genetics Perciformes - growth & development Perciformes - metabolism Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Scatophagus argus Sequence Homology, Amino Acid Vitellogenesis - physiology Vitellogenin Vitellogenins - genetics Vitellogenins - metabolism
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Zusammenfassung:	Highlights • erα, erβ1 and erβ2 partial cDNA of S. argus were cloned. • vtg-A, vtg-B and vtg-C partial cDNA of S. argus were cloned. • Ontogeny expression of three ers and three vtgs in female livers were performed. • The contribution of each Er to the regulation of different vtgs were determined.
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2017.03.002