Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

Dear Editor, The class 2/type Ⅱ clustered regularly interspaced short palindromic repeat （CRISPR）/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs （sgRNAs） to achieve mult...

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Veröffentlicht in:	Molecular plant 2017-07, Vol.10 (7), p.1011-1013
Hauptverfasser:	Wang, Mugui, Mao, Yanfei, Lu, Yuming, Tao, Xiaoping, Zhu, Jian-kang
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Clustered Regularly Interspaced Short Palindromic Repeats - genetics Francisella - chemistry Gene Editing - methods Oryza - genetics Prevotella - genetics RNAs 基因组多重植物细胞系统编辑重复序列
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Zusammenfassung:	Dear Editor, The class 2/type Ⅱ clustered regularly interspaced short palindromic repeat （CRISPR）/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs （sgRNAs） to achieve multiplex gene editing in plant cells.
ISSN:	1674-2052 1752-9867
DOI:	10.1016/j.molp.2017.03.001