Development and Validation of a Rapid LC-MS/MS Method for Simultaneous Determination of Kaempferol and Quercetin in Thespesia populnea Extract

Development and Validation of a Rapid LC-MS/MS Method for Simultaneous Determination of Kaempferol and Quercetin in Thespesia populnea Extract

In this study, a simple and rapid LC with tandem MS method was developed and validated for the simultaneous determination of kaempferol and quercetin in Thespesia populnea extract. The compounds were eluted using a Gemini C18 column (50 × 2.0 mm, 3 μm), with the mobile phase consisting of acetonitri...

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Veröffentlicht in:Journal of AOAC International 2017-07, Vol.100 (4), p.971-975
Hauptverfasser: Panchal, Hiteksha, Shah, Mamta B
Format: Artikel
Sprache:eng
Schlagworte:
Chemical properties
Chromatography, Liquid
Kaempferols - analysis
Liquid chromatography
Malvaceae
Malvaceae - chemistry
Mass spectrometry
Plant extracts
Plant Extracts - analysis
Quercetin - analysis
Tandem Mass Spectrometry
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Zusammenfassung:In this study, a simple and rapid LC with tandem MS method was developed and validated for the simultaneous determination of kaempferol and quercetin in Thespesia populnea extract. The compounds were eluted using a Gemini C18 column (50 × 2.0 mm, 3 μm), with the mobile phase consisting of acetonitrile-0.3% formic acid in water at the flow rate of 0.400 mL/min. The assay exhibited a linear dynamic range of 25-2500 ng/mL for both kaempferol and quercetin. The values for intra- and interday precision and accuracy were well within the generally accepted criteria for analytical methods (
ISSN:1060-3271
1944-7922
DOI:10.5740/jaoacint.16-0416