Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice

Summary Background Aloe vera is a traditional medical plant whose gel has been widely used in skin care. Previously, we have identified Aloe sterols from Aloe vera as active ingredients. This study investigated the protective effects of Aloe sterols without polysaccharides, against ultraviolet B (UVB)‐induced skin photoaging in mice using Aloe vera gel extract (AVGE) obtained by supercritical fluid extraction. Methods Aloe vera gel extract was supplemented in the diet (12 or 120 ppm), and HR‐1 hairless mice were exposed to UVB irradiation for 7 weeks. Skin measurements and histological and analytical studies were performed. Results Repeated UVB irradiation induced rough wrinkling of skin with water content reduction and hyperkeratosis. AVGE administration resulted in the significant improvement of UVB‐induced skin dryness, epidermal thickness, and wrinkle formation. The AVGE group also suppressed the degenerations of dermal collagen fibers and the appearance of cutaneous apoptosis cells induced by UVB. Furthermore, AVGE administration reduced the excess elevation of pro‐inflammatory cytokines (IL‐1β and TNF‐α) and matrix metalloproteinases (MMP‐2, MMP‐9, MMP‐12, and MMP‐13) in UVB‐exposed skin. Conclusion The dietary ingestion of Aloe sterols protected against chronic UVB damage in mouse skin, and our results suggest that Aloe sterols may prevent skin photoaging through the anti‐inflammation and MMP regulation.