Evaluation of the Illumina ForenSeq™ DNA Signature Prep Kit – MPS forensic application for the MiSeq FGx™ benchtop sequencer

Highlights • First commercial MPS kit on STR and SNP typing for forensics tested. • Strong reproducibility and sensitivity results – 136 markers with 0.05 ng input DNA. • Better mixture discrimination – decoupling of isometric alleles (same size, different sequence alleles). • Ancient DNA samples –...

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Veröffentlicht in:	Forensic science international : genetics 2017-05, Vol.28, p.188-194
Hauptverfasser:	Xavier, Catarina, Parson, Walther
Format:	Artikel
Sprache:	eng
Schlagworte:	Ancient DNA Chromosomes, Human, X Chromosomes, Human, Y DNA Fingerprinting Female Forensic Genetics Forensic science High-Throughput Nucleotide Sequencing - instrumentation Humans Male Massiv parallel sequencing Microsatellite Repeats Pathology Polymorphism, Single Nucleotide Reproducibility of Results Short tandem repeats Single nucleotide polymorphisms
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Beschreibung
Zusammenfassung:	Highlights • First commercial MPS kit on STR and SNP typing for forensics tested. • Strong reproducibility and sensitivity results – 136 markers with 0.05 ng input DNA. • Better mixture discrimination – decoupling of isometric alleles (same size, different sequence alleles). • Ancient DNA samples – 58.2% of profile typed as the lowest result
ISSN:	1872-4973 1878-0326
DOI:	10.1016/j.fsigen.2017.02.018