A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice

Gene-targeting technologies using sequence specific nucleases have been widely adopted to induce genetic modifications in both plant molecular biology research and crop improvement,of which generating targeted point mutation for gain-of-function phenotype is of great value.

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Bibliographische Detailangaben
Veröffentlicht in:	Science China. Life sciences 2017-05, Vol.60 (5), p.516-519
Hauptverfasser:	Ren, Bin, Yan, Fang, Kuang, Yongjie, Li, Na, Zhang, Dawei, Lin, Honghui, Zhou, Huanbin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biomedical and Life Sciences CRISPR CRISPR-Cas Systems DNA, Plant - genetics DNA, Plant - metabolism Gene Editing - methods Genes, Plant - genetics Genetic Variation Genome, Plant - genetics Letter to the Editor Life Sciences Oryza - genetics RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism 基因变异基因打靶技术基础工具包植物分子生物学水稻编辑诱导
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Beschreibung
Zusammenfassung:	Gene-targeting technologies using sequence specific nucleases have been widely adopted to induce genetic modifications in both plant molecular biology research and crop improvement,of which generating targeted point mutation for gain-of-function phenotype is of great value.
ISSN:	1674-7305 1869-1889
DOI:	10.1007/s11427-016-0406-x