In-depth transcriptome analysis of Larimichthys polyactis, de novo assembly, functional annotation

Larimichthys polyactis (small yellow croaker) is a commercially important marine benthic fish, and is the main catching target of fisheries in China, Japan and Korea. Researches across different fields have progressed the understanding of this species; however, no genomics studies of this fish have been reported so far. In this study, we performed de novo transcriptome sequencing of eight cDNA libraries from brain, gill, heart, intestine, liver, muscle, ovary, and testis tissues of L. polyactis. A total of 182,227,948 paired-end reads (>200bp) were generated, from which 134,439 transcripts were assembled. These transcripts yielded a total of 93,990 non-redundant transcripts (unigenes) that were assigned functional annotations based on BlastX searches (E-value