Difference in Protein Expression of Pinus tabulaeformis Induced by Dendrolimus tabulaeformis Feeding and Leaf-Cutting Stimulation

[Objective]This study aimed at detecting the protein expression differences and the protein base of defense reaction in Chinese pine(Pinus tabulaeformis) induced by leaf-cutting and different number pine caterpillar(Dendrolimus tabulaeformis) feeding stimulation in the natural environment.[Method]15-year-old Chinese pines with the similar growth potential were selected as the research object.The sample trees were treated with feeding stimulation by 10 or 30 pine caterpillars laid on the branch or with leaf-cutting,and the untreated trees served as the control in a Pinus tabulaeformisAmeniaca sibirica mixed forest in Pingquan County,Hebei Province.The protein expression differences of the Chinese pine were studied by dimensional electrophoresis method.[Result]Through the calculation of protein point expression,compared with control,after feeding with 10 pine caterpillars the up-regulated protein spots were 9,down-regulated protein spots were 4,new increased protein spots were 20,and disappeared protein spots w