A medically relevant capsular polysaccharide in Acinetobacter baumannii is a potential vaccine candidate

Abstract Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found t...

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Veröffentlicht in:Vaccine 2017-03, Vol.35 (10), p.1440-1447
Hauptverfasser: Yang, Feng-Ling, Lou, Tze-Chi, Kuo, Shu-Chen, Wu, Wan-Ling, Chern, Jeffy, Lee, Yi-Tzu, Chen, Shui-Tsung, Zou, Wei, Lin, Nien-Tsung, Wu, Shih-Hsiung
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Sprache:eng
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Zusammenfassung:Abstract Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found that CPS-induced antibody provided 55% protection against challenge in an animal model. The CPS-specific antibody reacted with the surface components of about 62% clinical isolates (342/554 strains) from cross-sectional and longitudinal studies by dot-immunoassay. Pulsed-field gel electrophoresis of positive strains showed the antibody covered different clonalites of A. baumannii clinical isolates. Meanwhile, using the CPS antibody as a probe, we found a number of outer membrane proteins bound to the antibody, including OmpA/motB, TonB-dependent receptor, and Omp38, indicating their association with CPS. These results might lead to the use of the capsular polysaccharide as a vaccine to prevent A. baumannii infection.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2017.01.060