Characterization of membrane progestin receptor α (mPRα) of the medaka and role in the induction of oocyte maturation
Oocyte maturation in medaka is induced by the maturation-inducing hormone (MIH) via its membrane receptor. The most likely candidates for the membrane receptor are membrane progestin receptors (mPRs). In order to characterize the mPRα subtype of medaka, a human cell line expressing the mPRα gene of...
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Veröffentlicht in: | Biomedical Research 2017/02/01, Vol.38(1), pp.79-87 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Oocyte maturation in medaka is induced by the maturation-inducing hormone (MIH) via its membrane receptor. The most likely candidates for the membrane receptor are membrane progestin receptors (mPRs). In order to characterize the mPRα subtype of medaka, a human cell line expressing the mPRα gene of medaka was established and its steroid binding property was assessed. The α subtype exhibited high binding affinity for 17,20β-DHP, the MIH in medaka. Treatment with a morpholino antisense oligonucleotide to mPRα blocked oocyte maturation in vivo. These results suggest that the medaka mPRα protein acts as an intermediary during MIH-induced oocyte maturation in medaka in a manner similar to that described previously for fish species. |
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ISSN: | 0388-6107 1880-313X |
DOI: | 10.2220/biomedres.38.79 |