Platelet count may impact on lysosomal acid lipase activity determination in dried blood spot

We aimed to evaluate the influence of white blood cell (WBC) and platelet (PLT) counts on dried blood spot (DBS)-determined lysosomal acid lipase (LAL) activity in a large group of healthy subjects. One-hundred-and-seventy-two healthy subjects aged ≥18 were enrolled. Complete clinical biochemistry a...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Clinical biochemistry 2017-08, Vol.50 (12), p.726-728
Hauptverfasser:	Vespasiani-Gentilucci, Umberto, D'Amico, Jessica, De Vincentis, Antonio, Tozzi, Giulia, Vorini, Ferruccio, Gallo, Paolo, Carotti, Simone, Valentini, Francesco, Galati, Giovanni, dell'Unto, Chiara, Piemonte, Fiorella, Picardi, Antonio
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Blood Chemical Analysis - standards Dried blood spot Dried Blood Spot Testing - standards Female Humans Leukocyte Count Lysosomal acid lipase Male Middle Aged Platelet Count Platelets Regression Analysis Sterol Esterase - blood White blood cells
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We aimed to evaluate the influence of white blood cell (WBC) and platelet (PLT) counts on dried blood spot (DBS)-determined lysosomal acid lipase (LAL) activity in a large group of healthy subjects. One-hundred-and-seventy-two healthy subjects aged ≥18 were enrolled. Complete clinical biochemistry and LAL activity in DBS were determined. In 35 subjects, WBCs and PLTs were isolated, and LAL activity was measured in both blood cell populations. Univariate and multivariate analyses to DBS-LAL activity were performed. Mean age of subjects was 44.8±17.2years, 43.6% were males, and mean DBS-LAL activity was normal (1.0±0.3nmol/spot/h). LAL activity in WBCs was significantly higher than in PLTs (458.9±133.6 vs 235.0±88.3nmol/mg/h, p
ISSN:	0009-9120 1873-2933
DOI:	10.1016/j.clinbiochem.2017.02.013