Expression of cytochromes P450 3A in mouse lung: effects of dexamethasone and pregnenolone 16α-carbonitrile

Expression of cytochromes P450 3A in mouse lung: effects of dexamethasone and pregnenolone 16α-carbonitrile

Expression of cytochromes P450 3A (CYP3A) has been reported in the lung, but its regulation has received little attention. In the present study, we assessed lung levels of Cyp3a mRNA, protein and activity in control mice and in mice treated with either dexamethasone (DEX), pregnenolone 16 alpha -car...

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Veröffentlicht in:Archives of toxicology 2003-03, Vol.77 (3), p.145-149
Hauptverfasser: HAAG, Muriel, FAUTREL, Alain, GUILLOUZO, André, FROSSARD, Nelly, PONS, Francoise
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Oxidoreductases
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Zusammenfassung:Expression of cytochromes P450 3A (CYP3A) has been reported in the lung, but its regulation has received little attention. In the present study, we assessed lung levels of Cyp3a mRNA, protein and activity in control mice and in mice treated with either dexamethasone (DEX), pregnenolone 16 alpha -carbonitrile (PCN) or a mixture of DEX+PCN. Lung expression of the pregnane X receptor (PXR) was also investigated. Constitutive levels of Cyp3a mRNA were found in the lung from control mice by polymerase chain reaction after reverse transcription of total RNA (RT-PCR). These levels were significantly increased (2.0-fold, P
ISSN:0340-5761
1432-0738
DOI:10.1007/s00204-002-0426-7