A Macrophage-Tropic HIV-1 That Expresses Green Fluorescent Protein and Infects Alveolar and Blood Monocyte-Derived Macrophages

A Macrophage-Tropic HIV-1 That Expresses Green Fluorescent Protein and Infects Alveolar and Blood Monocyte-Derived Macrophages

We describe the construction of a macrophage-tropic HIV-1 molecular clone, pNLAD8-EGFP, which expresses enhanced green fluorescent protein. We show that NLAD8-EGFP can infect monocyte-derived macrophages as well as alveolar macrophages. NLAD8-EGFP-infected macrophages can be easily and sensitively d...

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Veröffentlicht in:Journal of biomedical science 2002-12, Vol.9 (6), p.721-726
Hauptverfasser: Rich, Elizabeth A., Orenstein, Jan M., Jeang, Kuan-Teh
Format: Artikel
Sprache:eng
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Short Communication
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Zusammenfassung:We describe the construction of a macrophage-tropic HIV-1 molecular clone, pNLAD8-EGFP, which expresses enhanced green fluorescent protein. We show that NLAD8-EGFP can infect monocyte-derived macrophages as well as alveolar macrophages. NLAD8-EGFP-infected macrophages can be easily and sensitively detected based on the visualization of intracellular green fluorescent protein.
ISSN:1021-7770
1423-0127
DOI:10.1159/000067293