A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats
A simple, accurate, and reproducible high‐performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal stand...
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| Veröffentlicht in: | Biomedical chromatography 2017-03, Vol.31 (3), p.np-n/a |
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| creator | Abdelkawy, Khaled S. Balyshev, Mark E. Elbarbry, Fawzy |
| description | A simple, accurate, and reproducible high‐performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC‐18 T C18 column and an isocratic mobile phase consisting of 0.3% trichloroacetic acid in water and acetonitrile HPLC‐grade (50:50, v/v) run at a flow rate of 0.9 mL/min for 13 min. The UV detection wavelength was set at 254 nm. The method exhibited good linearity (R2 > 0.994) over the assayed concentration range (0.10–25 μg/mL) and demonstrated good intra‐day and inter‐day precision and accuracy (relative standard deviations and the deviation from predicted values were |
| doi_str_mv | 10.1002/bmc.3819 |
| format | Article |
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The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC‐18 T C18 column and an isocratic mobile phase consisting of 0.3% trichloroacetic acid in water and acetonitrile HPLC‐grade (50:50, v/v) run at a flow rate of 0.9 mL/min for 13 min. The UV detection wavelength was set at 254 nm. The method exhibited good linearity (R2 > 0.994) over the assayed concentration range (0.10–25 μg/mL) and demonstrated good intra‐day and inter‐day precision and accuracy (relative standard deviations and the deviation from predicted values were <20%). This method was also successfully applied for studying the pharmacokinetics of QR in rats following a single oral dose of QR to evaluate its pharmacokinetic parameters in rats.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.3819</identifier><identifier>PMID: 27555122</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Benzoquinones - blood ; Benzoquinones - pharmacokinetics ; Chromatography, High Pressure Liquid - methods ; HPLC ; Male ; pharmacokinetics ; quercetin ; Quercetin - blood ; Quercetin - pharmacokinetics ; Rats ; Reproducibility of Results ; thymoquinone ; validation</subject><ispartof>Biomedical chromatography, 2017-03, Vol.31 (3), p.np-n/a</ispartof><rights>Copyright © 2016 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4209-9fe25047d7604792a984fe41beceb3722fb74ab036476333a275637c800a17633</citedby><cites>FETCH-LOGICAL-c4209-9fe25047d7604792a984fe41beceb3722fb74ab036476333a275637c800a17633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.3819$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.3819$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27555122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abdelkawy, Khaled S.</creatorcontrib><creatorcontrib>Balyshev, Mark E.</creatorcontrib><creatorcontrib>Elbarbry, Fawzy</creatorcontrib><title>A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats</title><title>Biomedical chromatography</title><addtitle>Biomed Chromatogr</addtitle><description>A simple, accurate, and reproducible high‐performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC‐18 T C18 column and an isocratic mobile phase consisting of 0.3% trichloroacetic acid in water and acetonitrile HPLC‐grade (50:50, v/v) run at a flow rate of 0.9 mL/min for 13 min. The UV detection wavelength was set at 254 nm. The method exhibited good linearity (R2 > 0.994) over the assayed concentration range (0.10–25 μg/mL) and demonstrated good intra‐day and inter‐day precision and accuracy (relative standard deviations and the deviation from predicted values were <20%). This method was also successfully applied for studying the pharmacokinetics of QR in rats following a single oral dose of QR to evaluate its pharmacokinetic parameters in rats.</description><subject>Animals</subject><subject>Benzoquinones - blood</subject><subject>Benzoquinones - pharmacokinetics</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>HPLC</subject><subject>Male</subject><subject>pharmacokinetics</subject><subject>quercetin</subject><subject>Quercetin - blood</subject><subject>Quercetin - pharmacokinetics</subject><subject>Rats</subject><subject>Reproducibility of Results</subject><subject>thymoquinone</subject><subject>validation</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P3DAQhi1UBMuHxC9APvYSOraT2O5tWUFBWgQHOEeOM9GaJnGwvaD992S7lJ4qcfFInkePZuYl5IzBBQPgP-reXgjF9B6ZMdA6AwXsG5kBL3UmlNSH5CjGZwDQJZcH5JDLoigY5zPSz-mAb_TVdK4xCRt687Bc0B7Tyje09YGmFdIGE4beDSY5P1Df0pc1BovJDT_pfBw7Z3ed5GlM62ZDx5UJvbH-txsmykbqBhpMiidkvzVdxNOPekyerq8eFzfZ8v7X7WK-zGzOQWe6RV5ALhtZTq_mRqu8xZzVaLEWkvO2lrmpQZS5LIUQZtqnFNIqAMO2P8fk-847Bj_NGlPVu2ix68yAfh0rpkoleKG0-gJaaMmEyOEfaoOPMWBbjcH1JmwqBtU2h2rKodrmMKHnH9Z13WPzCf49_ARkO-DNdbj5r6i6vFv8Eb4DlcyQHw</recordid><startdate>201703</startdate><enddate>201703</enddate><creator>Abdelkawy, Khaled S.</creator><creator>Balyshev, Mark E.</creator><creator>Elbarbry, Fawzy</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201703</creationdate><title>A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats</title><author>Abdelkawy, Khaled S. ; Balyshev, Mark E. ; Elbarbry, Fawzy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4209-9fe25047d7604792a984fe41beceb3722fb74ab036476333a275637c800a17633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Benzoquinones - blood</topic><topic>Benzoquinones - pharmacokinetics</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>HPLC</topic><topic>Male</topic><topic>pharmacokinetics</topic><topic>quercetin</topic><topic>Quercetin - blood</topic><topic>Quercetin - pharmacokinetics</topic><topic>Rats</topic><topic>Reproducibility of Results</topic><topic>thymoquinone</topic><topic>validation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdelkawy, Khaled S.</creatorcontrib><creatorcontrib>Balyshev, Mark E.</creatorcontrib><creatorcontrib>Elbarbry, Fawzy</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdelkawy, Khaled S.</au><au>Balyshev, Mark E.</au><au>Elbarbry, Fawzy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed Chromatogr</addtitle><date>2017-03</date><risdate>2017</risdate><volume>31</volume><issue>3</issue><spage>np</spage><epage>n/a</epage><pages>np-n/a</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>A simple, accurate, and reproducible high‐performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC‐18 T C18 column and an isocratic mobile phase consisting of 0.3% trichloroacetic acid in water and acetonitrile HPLC‐grade (50:50, v/v) run at a flow rate of 0.9 mL/min for 13 min. The UV detection wavelength was set at 254 nm. The method exhibited good linearity (R2 > 0.994) over the assayed concentration range (0.10–25 μg/mL) and demonstrated good intra‐day and inter‐day precision and accuracy (relative standard deviations and the deviation from predicted values were <20%). This method was also successfully applied for studying the pharmacokinetics of QR in rats following a single oral dose of QR to evaluate its pharmacokinetic parameters in rats.</abstract><cop>England</cop><pmid>27555122</pmid><doi>10.1002/bmc.3819</doi><tpages>8</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Benzoquinones - blood Benzoquinones - pharmacokinetics Chromatography, High Pressure Liquid - methods HPLC Male pharmacokinetics quercetin Quercetin - blood Quercetin - pharmacokinetics Rats Reproducibility of Results thymoquinone validation |
| title | A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats |
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