Sinorhodobacter hungdaonensis sp. nov. isolated from activated sludge collected from a municipal wastewater treatment plant

A novel bacterium, strain L3 T , was isolated from an activated sludge sample retrieved from a municipal wastewater treatment plant in Huangdao, China. On the basis of 16S rRNA gene sequence similarity studies, strain L3 T was affiliated to the genus Sinorhodobacter , being most closely related to Sinorhodobacter ferrireducens (98.0 %). The 16S rRNA gene sequence similarity of strain L3 T to other related species, Thioclava atlantica DLFJ1-1 T (96.5 %), Rhodobacter capsulatus ATCC 11166 T (96.3 %), Paenirhodobacter enshiensis DW2-9 T (96.3 %) and Rhodobacter viridis JA737 T (96.0 %) is less than 96.5 %. Chemotaxonomic characterization further supported classification of the strain to the genus Sinorhodobacter . The major polar lipid profile consists of diphosphatidyglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids are C 18:1 ω7c (66.3 %), C 16:0 (12.9 %) and C 18:0 (8.0 %). The major quinone is Q-10. The G+C content of the genomic DNA of strain L3 T is 68.0 mol %. DNA–DNA relatedness value between L3 T and the closely related type strain S. ferrireducens SgZ-3 T was 35.2 %. Based on these results, a new species Sinorhodobacter huangdaonensis is proposed. The type strain is L3 T (= CGMCC 1.12963 T = KCTC 42823 T ).