Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay

A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% dia...

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Veröffentlicht in:	Molecular and cellular probes 2017-06, Vol.33, p.8-10
Hauptverfasser:	Chen, Jingfang, Zhang, Rusheng, Ou, Xinhua, Yao, Dong, Huang, Zheng, Li, Linzhi, Sun, Biancheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Viral - genetics Antigens, Viral - isolation & purification Coxsackievirus A10 Enterovirus Enterovirus - genetics Enterovirus - isolation & purification Enterovirus - pathogenicity Enterovirus Infections - diagnosis Enterovirus Infections - genetics Enterovirus Infections - virology Genotype Hand-foot-mouth disease Humans Molecular Diagnostic Techniques Real time RT-PCR
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Beschreibung
Zusammenfassung:	A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% diagnostic sensitivity in CV-A10 detection and 97.4% diagnostic sensitivity in other EVs were found. •A duplex real time RT-PCR assay for the detection of Enterovirus and Coxsackievirus A10 was developed.•The assay was specific and sensitive and had been evaluated in 115 clinical samples.•This method could be an useful alternative for routine surveillance of CV-A10 and other EVs.
ISSN:	0890-8508 1096-1194
DOI:	10.1016/j.mcp.2017.02.003