Effects of lipopolysaccharide-stimulated inflammation and pyrazole-mediated hepatocellular injury on mouse hepatic Cyp2a5 expression

Murine hepatic cytochrome P450 2a5 (Cyp2a5) is induced during hepatotoxicity and hepatitis, however, the specific regulatory mechanisms have not been determined. We compared the influence of acute inflammation elicited in vivo by bacterial endotoxin lipopolysaccharide (LPS) and liver injury caused b...

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Veröffentlicht in:	Toxicology (Amsterdam) 2003-03, Vol.184 (2), p.211-226
Hauptverfasser:	Gilmore, W.James, Hartmann, Georgy, Piquette-Miller, Micheline, Marriott, Jason, Kirby, Gordon M
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine Transaminase - blood Amyloid beta-Peptides - metabolism Analytical, structural and metabolic biochemistry Animals Aryl Hydrocarbon Hydroxylases - metabolism Biological and medical sciences Blotting, Northern Blotting, Western Carrier Proteins - metabolism Cell injury Chemical and Drug Induced Liver Injury - enzymology Chemical and Drug Induced Liver Injury - pathology Cytochrome P-450 CYP2A6 Cytochrome P450 Cytochrome P450 Family 2 Cytokines Cytokines - metabolism Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Gastroenterology. Liver. Pancreas. Abdomen Heat-Shock Proteins Hepatocytes - drug effects Hepatocytes - enzymology Hepatocytes - ultrastructure Immunohistochemistry Inflammation - chemically induced Inflammation - enzymology Interleukin-1 - pharmacology Interleukin-6 - pharmacology Lipopolysaccharide Lipopolysaccharides - pharmacology Liver - drug effects Liver - enzymology Liver - pathology Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Mice Mice, Inbred DBA Microscopy, Electron Mixed Function Oxygenases - metabolism Molecular Chaperones - metabolism Other diseases. Semiology Oxidoreductases Pyrazoles - toxicity Regulation
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Zusammenfassung:	Murine hepatic cytochrome P450 2a5 (Cyp2a5) is induced during hepatotoxicity and hepatitis, however, the specific regulatory mechanisms have not been determined. We compared the influence of acute inflammation elicited in vivo by bacterial endotoxin lipopolysaccharide (LPS) and liver injury caused by the hepatotoxin pyrazole on hepatic Cyp2a5 expression in mice. Pyrazole treatment resulted in statistically significant increases in levels of Cyp2a5 mRNA, protein and catalytic activity by 540, 273 and 711%, respectively ( P
ISSN:	0300-483X 1879-3185
DOI:	10.1016/S0300-483X(02)00581-4