Isolation and Identification of Antioxidant Peptides from Black Soybean Protein

The aim of this work was to isolate and identify antioxidant peptides from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and the fraction BSP-DA-c performed high antioxidant activity was further purified using consecutive methods o...

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Veröffentlicht in:Applied Mechanics and Materials 2014-08, Vol.618 (Materials, Machines and Development of Technologies for Industrial Production), p.298-302
Hauptverfasser: Zhang, Jian Ping, Chen, Shang Long, Wu, Yong Hua, Geng, Zhong Hua, Li, Yong, Liu, En Qi
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container_end_page 302
container_issue Materials, Machines and Development of Technologies for Industrial Production
container_start_page 298
container_title Applied Mechanics and Materials
container_volume 618
creator Zhang, Jian Ping
Chen, Shang Long
Wu, Yong Hua
Geng, Zhong Hua
Li, Yong
Liu, En Qi
description The aim of this work was to isolate and identify antioxidant peptides from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and the fraction BSP-DA-c performed high antioxidant activity was further purified using consecutive methods on Sephadex G-25 column and reversed phase high-performance liquid chromatography (RH-HPLC). Two highly purified antioxidant peptides SBP3 and BSPb were got, and their amino acid sequences were confirmed as Trp-Asn-Pro and Tyr-Asn-Ile by automated Edman degradation with a protein sequencer, respectively.
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subjects Amino acids
Antioxidants
Hydrolysates
Identification methods
Peptides
Polymers
Proteins
Soybeans
title Isolation and Identification of Antioxidant Peptides from Black Soybean Protein
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