Isolation and Identification of Antioxidant Peptides from Black Soybean Protein
The aim of this work was to isolate and identify antioxidant peptides from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and the fraction BSP-DA-c performed high antioxidant activity was further purified using consecutive methods o...
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Veröffentlicht in: | Applied Mechanics and Materials 2014-08, Vol.618 (Materials, Machines and Development of Technologies for Industrial Production), p.298-302 |
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container_issue | Materials, Machines and Development of Technologies for Industrial Production |
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creator | Zhang, Jian Ping Chen, Shang Long Wu, Yong Hua Geng, Zhong Hua Li, Yong Liu, En Qi |
description | The aim of this work was to isolate and identify antioxidant peptides from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and the fraction BSP-DA-c performed high antioxidant activity was further purified using consecutive methods on Sephadex G-25 column and reversed phase high-performance liquid chromatography (RH-HPLC). Two highly purified antioxidant peptides SBP3 and BSPb were got, and their amino acid sequences were confirmed as Trp-Asn-Pro and Tyr-Asn-Ile by automated Edman degradation with a protein sequencer, respectively. |
doi_str_mv | 10.4028/www.scientific.net/AMM.618.298 |
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Two highly purified antioxidant peptides SBP3 and BSPb were got, and their amino acid sequences were confirmed as Trp-Asn-Pro and Tyr-Asn-Ile by automated Edman degradation with a protein sequencer, respectively.</description><subject>Amino acids</subject><subject>Antioxidants</subject><subject>Hydrolysates</subject><subject>Identification methods</subject><subject>Peptides</subject><subject>Polymers</subject><subject>Proteins</subject><subject>Soybeans</subject><issn>1660-9336</issn><issn>1662-7482</issn><issn>1662-7482</issn><isbn>3038352047</isbn><isbn>9783038352044</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNkF1LHDEUQEOtoKv9DwOC-DKz-ZpM5kVcF9suKC7UPofMzA2NziZrkmXrv2_WFSw--RSSezi5HITOCa44pnK63W6r2FtwyRrbVw7SdHZ3VwkiK9rKL-iYCEHLhkt6gCYMM8lqinnz9XWAy5YxcYQmMT5iLDjh8hjdL6IfdbLeFdoNxWJ4c--fvClm-e7_2kG7VCxhnewAsTDBr4rrUfdPxS__0oF2xTL4BNadokOjxwjf3s4T9Pv7zcP8Z3l7_2Mxn92WPaMylR3vJOk6QcA0rMaa1CYvN9SdAWBcmIE0Bhpo644JaA1IjofBCF5T0mpoCTtBF3vvOvjnDcSkVjb2MI7agd9ERWSGm4bWO_TsA_roN8Hl7TJF67YVkjeZutxTffAxBjBqHexKhxdFsNrFVzm-eo-vcnyV46scX-X4WXC1F6SgXUzQ__nvn88p_gEZe5R6</recordid><startdate>20140818</startdate><enddate>20140818</enddate><creator>Zhang, Jian Ping</creator><creator>Chen, Shang Long</creator><creator>Wu, Yong Hua</creator><creator>Geng, Zhong Hua</creator><creator>Li, Yong</creator><creator>Liu, En Qi</creator><general>Trans Tech Publications Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BFMQW</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>KR7</scope><scope>L6V</scope><scope>M7S</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20140818</creationdate><title>Isolation and Identification of Antioxidant Peptides from Black Soybean Protein</title><author>Zhang, Jian Ping ; 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subjects | Amino acids Antioxidants Hydrolysates Identification methods Peptides Polymers Proteins Soybeans |
title | Isolation and Identification of Antioxidant Peptides from Black Soybean Protein |
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