Isolation and Identification of Antioxidant Peptides from Black Soybean Protein

Isolation and Identification of Antioxidant Peptides from Black Soybean Protein

The aim of this work was to isolate and identify antioxidant peptides from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and the fraction BSP-DA-c performed high antioxidant activity was further purified using consecutive methods o...

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Veröffentlicht in:Applied Mechanics and Materials 2014-08, Vol.618 (Materials, Machines and Development of Technologies for Industrial Production), p.298-302
Hauptverfasser: Liu, En Qi, Chen, Shang Long, Geng, Zhong Hua, Zhang, Jian Ping, Li, Yong, Wu, Yong Hua
Format: Artikel
Sprache:eng
Schlagworte:
Amino acids
Antioxidants
Hydrolysates
Identification methods
Peptides
Polymers
Proteins
Soybeans
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Zusammenfassung:The aim of this work was to isolate and identify antioxidant peptides from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and the fraction BSP-DA-c performed high antioxidant activity was further purified using consecutive methods on Sephadex G-25 column and reversed phase high-performance liquid chromatography (RH-HPLC). Two highly purified antioxidant peptides SBP3 and BSPb were got, and their amino acid sequences were confirmed as Trp-Asn-Pro and Tyr-Asn-Ile by automated Edman degradation with a protein sequencer, respectively.
ISSN:1660-9336
1662-7482
1662-7482
DOI:10.4028/www.scientific.net/AMM.618.298