An efficient strategy to assemble water soluble histidine-perylene diimide and graphene oxide for the detection of PPi in physiological conditions and in vitro
A strategy to develop water soluble, biocompatible nanocomposite probe for the detection of pyrophosphate (PPi) in physiological conditions and in in vitro live melanoma cancer cells (B16F10) is reported. The self-assembled nanocomposite probe comprised of amino acid (histidine) functionalized peryl...
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Veröffentlicht in: | Biosensors & bioelectronics 2017-03, Vol.89 (Pt 1), p.636-644 |
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Zusammenfassung: | A strategy to develop water soluble, biocompatible nanocomposite probe for the detection of pyrophosphate (PPi) in physiological conditions and in in vitro live melanoma cancer cells (B16F10) is reported. The self-assembled nanocomposite probe comprised of amino acid (histidine) functionalized perylenediimide (PDI-HIS), copper ion and graphene oxide (GO) and that could be utilized as a highly effective sensing platform in biological conditions and cellular environment via fluorescence “turn-on” for PPi detection. This controlled fabrication of metal organic self-assembled spheres along with GO proved very valuable for the detection of PPi in unprecedented sensitivity over other competing ions. The PDI-HIS–Cu–GO (PCG) nanocomposite sensor provides a unique platform for the fluorogenic detection of PPi having a very low limit of detection (LOD) of 0.60×10−7M based on the strong affinity (1.0×106M−1) between the copper complex of PDI-HIS receptor and PPi. The intracellular detection of PPi using PCG also carried out in B16F10 cells where >10 times observed as compared to the PDI-HIS+Cu2+ complex. Thus early cancer detection via PPi recognition in physiological conditions and in live cells was possible using PCG. Furthermore, the fabrication of PDI-HIS and PCG with PVA hydrogel films and on thin layer chromatography plates demonstrated the practical utility for the detection of PPi anions by “off–on” response rapidly in a label free manner.
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•A new biosensing platform for detection of Cu2+ ion and cancer biomarker PPi is constructed.•PDI-HIS with Cu2+ and graphene oxide (PCG) was used for recognition.•Intracellular detection of PPi using PCG was also carried out in B16F10 cells.•Label-free fabrication of PDI-HIS and PCG with PVA hydrogel films and on TLC plates demonstrated. |
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ISSN: | 0956-5663 1873-4235 |
DOI: | 10.1016/j.bios.2015.12.036 |