A one-step multiplex PCR to identify Klebsiella pneumoniae , Klebsiella variicola and Klebsiella quasipneumoniae in the clinical routine

Abstract Klebsiella pneumoniae , Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, LEN and OKP and their flanking gene ( deo R). Since this scheme focus...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2017-04, Vol.87 (4), p.315-317
Hauptverfasser:	Fonseca, Erica Lourenço, da Veiga Ramos, Nilceia, Nascimento Andrade, Bruno G, Morais, Lena L.C.S, Abanto Marin, Michel F, Vicente, Ana Carolina P
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-Lactamases - genetics blaLEN blaOKP blaSHV Class A β-lactamase DNA, Bacterial - genetics Humans Infectious Disease Internal Medicine Klebsiella - genetics Klebsiella Infections - microbiology Klebsiella pneumoniae - genetics Klebsiella species Microbial Sensitivity Tests - methods Molecular identification Multiplex Polymerase Chain Reaction - methods
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Zusammenfassung:	Abstract Klebsiella pneumoniae , Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, LEN and OKP and their flanking gene ( deo R). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2017.01.005