Routine Identification Of Nocardia Species By Maldi-Tof Mass Spectrometry

Abstract We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust MALDI-TOF MS database leads to 94% accurate identification to the species level on...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2017-01, Vol.87 (1), p.7-10
Hauptverfasser:	Girard, Victoria, Mailler, Sandrine, Polsinelli, Sophie, Jacob, Delphine, Saccomani, Marie Christine, Celliere, Beatrice, Monnin, Valerie, van Belkum, Alex, Hagen, Ferry, Meis, Jacques F, Durand, Géraldine
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological Techniques - methods Humans Infectious Disease Internal Medicine MALDI TOF MS Nocardia Nocardia - chemistry Nocardia - classification Nocardia Infections - diagnosis Routine diagnostic microbiology Species identification Specimen Handling - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Zusammenfassung:	Abstract We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust MALDI-TOF MS database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2016.09.024