A rapid and sensitive method for the screening of DNA intercalating antibiotics

An improved, rapid and inexpensive gel mobility shift assay was developed for the screening of anthracycline antibiotics. The assay based on the intercalation activity of these molecules into dsDNA was used to assess the activity of partially purified antibiotics. Detection limits were of 0.1 ng ml^...

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Veröffentlicht in:	Biotechnology letters 2002-11, Vol.24 (21), p.1807-1813
Hauptverfasser:	FURLAN, Renata Ligia A, GARRIDO, Leandro M, BRUMATTI, Gabriela, AMARANTE-MENDES, Gustavo P, MARTINS, Renata A, FACCIOTTI, Maria Candida R, PADILLA, Gabriel
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics Biological and medical sciences Biotechnology Detection limits Fermentation Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Various methods and equipments
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Zusammenfassung:	An improved, rapid and inexpensive gel mobility shift assay was developed for the screening of anthracycline antibiotics. The assay based on the intercalation activity of these molecules into dsDNA was used to assess the activity of partially purified antibiotics. Detection limits were of 0.1 ng ml^sup -1^ with an average run time of 2 h. The assay is potentially useful for high throughput screening in bioprospecting, for monitoring fermentation production phases and downstream purification process.[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1020633909762