Improved recovery of pathogenic Vibrio parahaemolyticus from oysters using colony hybridization following enrichment

The traditional streak plating and alternative spread-plating methods were compared for detection of pathogenic Vibrio parahaemolyticus (Vp) in oyster enrichments. We found the alternative method to be more efficient: it was quicker (2d vs. 3d) and had a significantly ( p

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2003-02, Vol.52 (2), p.273-277
Hauptverfasser:	Nordstrom, Jessica L, DePaola, Angelo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Colony Count, Microbial - methods Crassostrea Culture Media DNA probe Fundamental and applied biological sciences. Psychology Hemolysin Proteins - analysis Marine Microbiology Nucleic Acid Hybridization Ostreidae - microbiology Sensitivity and Specificity tdh gene Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - isolation & purification Vibrio parahaemolyticus - pathogenicity
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Beschreibung
Zusammenfassung:	The traditional streak plating and alternative spread-plating methods were compared for detection of pathogenic Vibrio parahaemolyticus (Vp) in oyster enrichments. We found the alternative method to be more efficient: it was quicker (2d vs. 3d) and had a significantly ( p
ISSN:	0167-7012 1872-8359
DOI:	10.1016/S0167-7012(02)00188-4