Custom database development and biomarker discovery methods for MALDI-TOF mass spectrometry-based identification of high-consequence bacterial pathogens

A high-quality custom database of MALDI-TOF mass spectral profiles was developed with the goal of improving clinical diagnostic identification of high-consequence bacterial pathogens. A biomarker discovery method is presented for identifying and evaluating MALDI-TOF MS spectra to potentially differe...

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Veröffentlicht in:	Journal of microbiological methods 2017-03, Vol.134, p.54-57
Hauptverfasser:	Tracz, Dobryan M., Tyler, Andrea D., Cunningham, Ian, Antonation, Kym S., Corbett, Cindi R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus - genetics Bacillus - isolation & purification Bacteria - genetics Bacteria - isolation & purification Bacteria - pathogenicity Bacterial Infections - diagnosis Bacterial Infections - microbiology Bacterial Proteins - isolation & purification Bacterial Typing Techniques Biological Warfare Agents Biomarkers - analysis Bioterrorism bacterial pathogens Biothreat Brucella - genetics Brucella - isolation & purification Databases, Nucleic Acid Genus Yersinia, Bacillus, Brucella Humans MALDI-TOF mass spectrometry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards Yersinia - genetics Yersinia - isolation & purification
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Beschreibung
Zusammenfassung:	A high-quality custom database of MALDI-TOF mass spectral profiles was developed with the goal of improving clinical diagnostic identification of high-consequence bacterial pathogens. A biomarker discovery method is presented for identifying and evaluating MALDI-TOF MS spectra to potentially differentiate biothreat bacteria from less-pathogenic near-neighbour species.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2017.01.009