Cloth-based hybridization array system for detection of transgenic soy and corn by multiplex polymerase chain reaction

A cloth-based hybridization array system (CHAS) was developed for detection of amplicons generated in a multiplex PCR targeting transgenic 35S and NOS sequences, as well as corn invertase and soy lectin genes. The CHAS provided confirmation of each amplicon on the basis of hybridization with specifi...

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Veröffentlicht in:	Biotechnology letters 2002-09, Vol.24 (17), p.1407-1411
Hauptverfasser:	RINK, Burton W, PHILLIPPE, Lucille M, VARY, Neil
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Corn Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Hybridization Methods. Procedures. Technologies Microbiology Transgenic animals and transgenic plants Transgenic plants
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Zusammenfassung:	A cloth-based hybridization array system (CHAS) was developed for detection of amplicons generated in a multiplex PCR targeting transgenic 35S and NOS sequences, as well as corn invertase and soy lectin genes. The CHAS provided confirmation of each amplicon on the basis of hybridization with specific capture probes, and enabled ease of discrimination of the multiplex PCR products by visualization of the amplicons on the array.[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1019894330731