A Technique for Rapid Micropropagation of Solanum surattense Burm. f

In Solanum surattense, a medicinally important member of family Solanaceae, rapid micropropagation and direct organogenesis was achieved from shoot tip and leaf explants, respectively. Both leaf and shoot tips differentiated into number of small shoots without apparently forming any callus. Average 180-200 adventitious shoots were produced from a single explant on MS basal medium fortified with BAP and kinetin individually and in combination. Caulogenesis was followed by rhizogenesis on the same medium without phytohormone. In vitro regenerated plantlets were healthy with dark green leaves and attained a length of 5-10 cm in 10 weeks. The post-transplantation survival rate in the field was 80 plus or minus 5% without any seasonal constraints.