Closed nutrient recycling via microbial catabolism in an eco-engineered self regenerating mixed anaerobic microbiome for hydrogenotrophic methanogenesis

[Display omitted] •A methanation biocatalyst was observed to be capable of self-regeneration.•Self-regeneration was accomplished by creating a closed nutrient ecosystem.•pH of the media was controlled solely by the amount of CO2 entering the reactor. A novel eco-engineered mixed anaerobic culture wa...

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Veröffentlicht in:Bioresource technology 2017-03, Vol.227, p.93-101
Hauptverfasser: Savvas, Savvas, Donnelly, Joanne, Patterson, Tim, Dinsdale, Richard, Esteves, Sandra R.
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Sprache:eng
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Zusammenfassung:[Display omitted] •A methanation biocatalyst was observed to be capable of self-regeneration.•Self-regeneration was accomplished by creating a closed nutrient ecosystem.•pH of the media was controlled solely by the amount of CO2 entering the reactor. A novel eco-engineered mixed anaerobic culture was successfully demonstrated for the first time to be capable of continuous regeneration in nutrient limiting conditions. Microbial catabolism has been found to support a closed system of nutrients able to enrich a culture of lithotrophic methanogens and provide microbial cell recycling. After enrichment, the hydrogenotrophic species was the dominating methanogens while a bacterial substratum was responsible for the redistribution of nutrients. q-PCR results indicated that 7% of the total population was responsible for the direct conversion of the gases. The efficiency of H2/CO2 conversion to CH4 reached 100% at a gassing rate of above 60v/v/d. The pH of the culture media was effectively sustained at optimal levels (pH 7–8) through a buffering system created by the dissolved CO2. The novel approach can reduce the process nutrient/metal requirement and enhance the environmental and financial performance of hydrogenotrophic methanogenesis for renewable energy storage.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.12.052